In the past several decades, geospatial analysis based on geographic information systems (GIS) became an important methodology and technology in public health studies and practices. This type of application has been widely adopted in developed countries, and also gets popular in China. This course introduces basic concepts, theories, methodologies, and technologies of this new interdisciplinary area. The course will include three (3) sections. The first section covers fundamentals of GIS, including spatial database construction, operations, and basic spatial analyses. The second section is about mapping diseases and detecting disease-environment associations, covering basic statistical and cartographical knowledge involved in these practices. The third section is about assessment of spatial access to healthcare services, as well as associated social justice and disparity issues. Each section includes a lecture and a lab exercise. The course will use ArcGIS as the basic GIS software, and ArcHealth (developed by the instructor) as the special toolset for health applications. After taking the course, the students should be able to understand the basics of GIS-based spatial analysis and how to apply them to public health issues. Likely, they will develop ideas on how to use what they learn in the course in their own studies.