The course is an introduction to the fabrication of microfluidic platforms via 3D printing technologies. The course will briefly touch upon conventional microfluidic fabrication technologies (i.e. soft-lithography/cleanroom). Throughout the course, we will place an emphasis on bioanalytical microfluidic system applications and their fabrication via 3D printing. We will introduce transport phenomena relevant to microfluidic flow. Successful completion of the course will prepare graduate students and undergraduate students to develop a theoretical understanding of microfluidics, and critically assess academic and industrial developments in these areas. Topics include: microfabrication via 3D printing, advantages and limitations of different fabrication approaches, low Reynolds number fluid mechanics, diffusion phenomena. Applications focus on biological systems including point-of-care health diagnostics (yet transferrable to other fields including environmental monitoring etc.). We will (i) introduce the need for miniaturized systems in biology and medicine, (ii) introduce the basics of microscale manipulation of cells and biological agents employing the fundamentals of microscale behaviors of fluids and mechanical systems, and (iii) expose the students to central design considerations and emerging applications of microfluidic technologies in biology and medicine.